Heterogeneity of infectious bronchitis virus grown in eggs
Identifieur interne : 001776 ( Main/Exploration ); précédent : 001775; suivant : 001777Heterogeneity of infectious bronchitis virus grown in eggs
Auteurs : M. S. Collins [Royaume-Uni] ; D. J. Alexander [Royaume-Uni] ; J. W. Harkness [Royaume-Uni]Source :
- Archives of Virology [ 0304-8608 ] ; 1976-03-01.
English descriptors
- Teeft :
- Academic press, Allantoic fluid, Amorphous material, Avian, Bronchitis, Bronchitis virus, Buoyant density, Cellular material, Complement fixation test, Continuous sucrose gradients, Densest peak, Density gradient peaks, Different preparations, Discontinuous density gradients, Discontinuous gradient, Distinct peaks, Electron microscopy, Fertile chicken eggs, Fraction number, Great britain, Heterogeneity, Infectious bronchitis virus, Infectivity, Isopyenie separation, Large particles, Lipid composition, Major polypeptides, Minor polypeptides, Newcastle disease virus, Other peaks, Peak, Pleomorphic virus particles, Polypeptide, Relative migration, Similar results, Strain beaudette, Sucrose, Sucrose density gradients, Sucrose solutions, Virol, Virology, Virus, Virus particles.
Abstract
Summary: Egg-grown infectious bronchitis virus, strain Beaudette, was concentrated and centrifuged on sucrose density gradients to separate the virus into five peaks with densities of 1.144, 1.160, 1.172, 1.191 and 1.218 g/cm3. All peaks retained infectivity, complement fixation activity and were labelled with3H-uridine. Morphologically the densest peak consisted of very large virus particles and amorphous material, the other peaks consisted of mainly intact particles although small differences in size and pleomorphism were seen. Polyacrylamide gel electrophoresis of material from the density gradient peaks revealed four major polypeptides and at least 10 minor polypeptides. The proportions of the polypeptides were approximately similar for all peaks with the exception of the densest peak in which the major polypeptides were greatly reduced. The four major polypeptides had approximate molecular weights of 1. 52,000, 2. 45,000, 3. 34,000, 4. 32,000. The major polypeptides 1 and 4 were shown to be glycosylated as were two of the minor polypeptides.
Url:
DOI: 10.1007/BF01318001
Affiliations:
Links toward previous steps (curation, corpus...)
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Le document en format XML
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S." last="Collins">M. S. Collins</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Poultry Department, Central Veterinary Laboratory, New Haw, Weybridge, Surrey</wicri:regionArea><wicri:noRegion>Surrey</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Virology Department, Central Veterinary Laboratory, New Haw, Weybridge, Surrey</wicri:regionArea><wicri:noRegion>Surrey</wicri:noRegion></affiliation></author><author><name sortKey="Alexander, D J" sort="Alexander, D J" uniqKey="Alexander D" first="D. J." last="Alexander">D. J. 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Harkness</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Virology Department, Central Veterinary Laboratory, New Haw, Weybridge, Surrey</wicri:regionArea><wicri:noRegion>Surrey</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="abbrev">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="1976-03-01">1976-03-01</date><biblScope unit="volume">50</biblScope><biblScope unit="issue">1-2</biblScope><biblScope unit="page" from="55">55</biblScope><biblScope unit="page" to="72">72</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Allantoic fluid</term><term>Amorphous material</term><term>Avian</term><term>Bronchitis</term><term>Bronchitis virus</term><term>Buoyant density</term><term>Cellular material</term><term>Complement fixation test</term><term>Continuous sucrose gradients</term><term>Densest peak</term><term>Density gradient peaks</term><term>Different preparations</term><term>Discontinuous density gradients</term><term>Discontinuous gradient</term><term>Distinct peaks</term><term>Electron microscopy</term><term>Fertile chicken eggs</term><term>Fraction number</term><term>Great britain</term><term>Heterogeneity</term><term>Infectious bronchitis virus</term><term>Infectivity</term><term>Isopyenie separation</term><term>Large particles</term><term>Lipid composition</term><term>Major polypeptides</term><term>Minor polypeptides</term><term>Newcastle disease virus</term><term>Other peaks</term><term>Peak</term><term>Pleomorphic virus particles</term><term>Polypeptide</term><term>Relative migration</term><term>Similar results</term><term>Strain beaudette</term><term>Sucrose</term><term>Sucrose density gradients</term><term>Sucrose solutions</term><term>Virol</term><term>Virology</term><term>Virus</term><term>Virus particles</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: Egg-grown infectious bronchitis virus, strain Beaudette, was concentrated and centrifuged on sucrose density gradients to separate the virus into five peaks with densities of 1.144, 1.160, 1.172, 1.191 and 1.218 g/cm3. All peaks retained infectivity, complement fixation activity and were labelled with3H-uridine. Morphologically the densest peak consisted of very large virus particles and amorphous material, the other peaks consisted of mainly intact particles although small differences in size and pleomorphism were seen. Polyacrylamide gel electrophoresis of material from the density gradient peaks revealed four major polypeptides and at least 10 minor polypeptides. The proportions of the polypeptides were approximately similar for all peaks with the exception of the densest peak in which the major polypeptides were greatly reduced. The four major polypeptides had approximate molecular weights of 1. 52,000, 2. 45,000, 3. 34,000, 4. 32,000. The major polypeptides 1 and 4 were shown to be glycosylated as were two of the minor polypeptides.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Collins, M S" sort="Collins, M S" uniqKey="Collins M" first="M. S." last="Collins">M. S. Collins</name></noRegion><name sortKey="Alexander, D J" sort="Alexander, D J" uniqKey="Alexander D" first="D. J." last="Alexander">D. J. Alexander</name><name sortKey="Alexander, D J" sort="Alexander, D J" uniqKey="Alexander D" first="D. J." last="Alexander">D. J. Alexander</name><name sortKey="Collins, M S" sort="Collins, M S" uniqKey="Collins M" first="M. S." last="Collins">M. S. Collins</name><name sortKey="Harkness, J W" sort="Harkness, J W" uniqKey="Harkness J" first="J. W." last="Harkness">J. W. Harkness</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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